When taking multiple exposures, don't forget to use a tripod, and in this instance I would even turn off IS to make sure it wouldn't drift a pixel or two between shots. Use your self-timer or a USB remote release too, just so you don't move it a bit with your hand on the camera. With IS set to ON just the camera's own slight vibrations might be enough to make it shift a bit after each shot. This way you won't loose any detail by merging misaligned data.
exposure time was 1/320th of a second, iso 1600. I just held the trigger down in continuous mode for 25 pictures. didnt take long, mabe a minute or 2.
I notice crisper details in your noise-image examples, so I suspect this is what caused the loss of finer details in the merged results. In fact, if I load both images up into separate tabs in my browser and then click between them (using it like a "blink comparator", like what's used in astrophotography to watch for the smallest things that move between images), I can actually see the whole image shift from original to merged results. Confirming my suspicions.
I suppose your goal is noise reduction, then why do you use high(est) ISO rather than lowest ??
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